DNA

Part:BBa_K259008:Design

Designed by: Petros Mina   Group: iGEM09_BCCS-Bristol   (2009-10-13)


BioScaffold Linker - Removes Stop Codons & scars & replaces with a Gly-Ser linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CspCI enzyme has different variations in the distance of restricting DNA from the recognition site.This biobrick is almost identical to part BBa_K259004 but modified to account for this discrepancy.


Source

This part is completely designed de novo. This sequence does not occur naturally.

References